XXXIX WEB CONFERENCE GIC 2021

Lunedì 14 giugno

14.00
Apertura della Conferenza
Giovanni D’Arena (Salerno), Raffaele De Vita (Roma), Giuliano Mazzini (Pavia)

14.10 - 14.55
Sessione di Presentazione e Discussione Comunicazioni Poster Ematologia
Moderatori: Giovanni Rossi (San Giovanni Rotondo), Giulia Scalia (Napoli)

MORE THAN A SURFACE MARKER: THE RELEVANCE OF CD200 SERUM LEVELS IN PREDICTING PROGNOSIS OF CHRONIC LYMPHOCYTIC
LEUKEMIA
D’Arena G.¹, Vitale C.², Coscia M.², Lamorte D.³, Pietrantuono G.⁴, Perutelli F.², D’Auria F.⁵, , Valvano T.⁵, Tomassi S.⁶, Griggio V.², Jones R.², Di Leo A.⁷, Feola G.⁸, Guida G.⁸, Mansueto G.⁴, Villano O.⁴, D’Agostino S.⁴, De Feo V.⁹, Calapai F.¹⁰, Mannucci G.¹¹, Efremov D.¹², Laurenti L.⁶, (giovannidarena@libero.it)
1. Hematology, P.O. S. Luca, ASL Salerno;
2. Hematology, Department of Molecular Biotechnology and Health Sciences, A.O.U. Città della Salute e della Scienza di Torino, University of Torino;
3. Laboratory of Pre-Clinical and Translational Research, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
4. Hematology and Stem Cell Transplantation Unit, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
5. Laboratory of Clinical and Advanced Diagnostics, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
6. Hematology Unit, Catholic University of Sacred Hearth, Rome, 7. Ortopedics Unit, P.O. S. Luca ASL Salerno;
8. SIT, P.O. S. Luca, ASL Salerno, 9. Department of Pharmaceutical and Biomedical Sciences, University of Salerno;
10. Department of Clinical, Biological and Pharmaceutical in Environmental Sciences, University of Messina:
11. Department of Biomedical, Dental, Morphological and Functional Imaging Sciencies, University of Messina;
12. Molecular Hematology, International Center for Genetic Engineering and Biotechnology, Trieste.

WHAT DOES ATYPICAL CHRONIC LYMPHOCYTIC LEUKEMIA REALLY MEANS? MORPHOLOGY VS IMMUNOPHENOTYPE
D’Arena G.¹, D’Auria F.², Statuto T.², Valvano T.², Mansueto G.³, Villani O.³, D’Agostino S.³, Pietrantuono G.³
1. Hematology, P.O. S. Luca, ASL Salerno;
2. Laboratory of Clinical Research and Advanced Diagnostics, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
3. Hematology and Stem Cell Transplantation Unit, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture - giovannidarena@libero.it

APPLICATION OF FLOW CYTOMETRY FOR THE DETECTION OF THE ABUSE OF BLOOD TRANSFUSIONS IN SPORT DOPING: STATE OF THE ART AND NEW PERSPECTIVES
Francesco Donati¹, Giorgia Morgan Biasini¹, Livia Concetti¹, Xavier de la Torre¹, Francesco Botrè^1,2*
1. Laboratorio Antidoping, Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome Italy;
2. REDs - Research and Expertise in anti-Doping sciences, ISSUL - Institute of Sports Science, University of Lausanne, Switzerland

A NEW NON-INVASIVE METHOD FOR ISOLATION OF CIRCULATING EXTRACELLULAR VESICLES AND EVALUATION OF ITS SUITABILITY FOR
HEMATOLOGICAL MALIGNANCY BIOMARKER DISCOVERY
Laurenzana I.¹, Trino S.¹, Lamorte D.¹, Girasole M.², Dinarelli S.², De Stradis A.³, Grieco V.⁴, Maietti M.⁵, Traficante A.⁵, StatutoT.⁴, Villani O.⁶, Musto P.^6,7, Sgambato A.⁸, De Luca L.⁴, Caivano A.⁴
1. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Preclinical and Translational Research, Rionero in Vulture, PZ, IT;
2. National Research Council (CNR), Institute for the Study of the Structure of Matter, Rome, Italy;
3. National Research Council (CNR), Institute for Sustainable Plant Protection, Bari, Italy;
4. IRCCS-CROB, Laboratory of Clinical Research and Advanced Diagnostics, Rionero in Vulture, PZ, Italy;
5. IRCCS-CROB, Unit of Clinical Pathology, Rionero in Vulture, PZ, Italy;
6. IRCCS-CROB, Hematology and Stem Cell Transplantation Unit, Rionero in Vulture, PZ, Italy;
7. “Aldo Moro” University School of Medicine and Unit of Hematology and Stem Cell Transplantation, Policlinico, Department of Emergency and Organ Transplantation, Bari, Italy
8. IRCCS-CROB, Scientific Direction, Rionero in Vulture, PZ, Italy
ilaria.laurenzana@crob.it

CYTOFLUORIMETRIC ANALYSIS OF TWO BONE MARROW COLLECTION SYSTEMS AND THE IMPORTANCE OF CLINICAL RESULTS
Stefano Papini¹, Cristina Borrelli¹, Lella Petrella¹, Mara Fanelli¹, Maria Pia Petrilli¹, Pietro Modugno¹, Veronica Picone¹, Andrea Mazzanti², Pietro Ornati², Enrico Maria Centritto¹ and Eugenio Caradonna¹
1. Gemelli Molise -Campobasso;
2. IRCSS Maugeri - Pavia.

CYTOKINE ANALYSIS IN PLATELET RICH PLASMA OF PATIENTS WITH OSTEOARTHRITIS VS HEALTHY SUBJECTS
Stefano Papini¹, Mara Fanelli¹, Lella Petrella¹, Cristina Borrelli¹, Domenico Speciale², Antonio San Martino², Emilio Serlenga³, Andrea Mazzanti⁴, Pietro Ornati⁴ and Eugenio Caradonna¹
1. Gemelli Molise Campobasso;
2. Clinica San Camillo Taranto;
3. Presidio Ospedaliero SS. Annunziata Taranto;
4. IRCSS Maugeri Pavia.

PREMIO DI STUDIO GIC “GIUSEPPE BASSO”
MUSASHI-2 SUSTAINS THE GROWTH OF MLL-REARRANGED ALL AND IT IS INVOLVED IN GLUCOCORTICOIDS RESISTANCE
G. Passafari1², L. Valsecchi1², M. Mauri², R. Piazza², A. Biondi1², G. Cazzaniga1², M. Bardini¹
1. Fondazione M. Tettamanti, Clinica Pediatrica, Monza;
2. Università di Milano-Bicocca, Dipartimento di Medicina e Chirurgia, Monza.

PREMIO DI STUDIO GIC “GIUSEPPE BASSO”
FUNCTIONAL CHARACTERIZATION OF GENETIC VARIANTS, IDENTIFIED THROUGH NEXT GENERATION SEQUENCING, WITH A ROLE IN THE
PREDISPOSITION TO PEDIATRIC HEMATONCOLOGICAL DISEASES
S. Rebellato, C. Saitta, L. Bettini, G. Giudici, N. Panini, E. Erba, A. Biondi, G. Fazio, G. Cazzaniga
Centro Ricerca Tettamanti, Clinica Pediatrica, Università di Milano Bicocca, Fondazione MBBM, Monza

CIRCULATING REGULATORY T-CELL NUMBER DOES NOT PREDICT PROGNOSIS OF MONOCLONAL GAMMOPATHIES OF UNCERTAIN SIGNIFICANCE
Valvano L.^1, Calice G. ^2, Statuto T.¹, Pietrantuono G.³, Mansueto G.³, Villani O.³, Marano L.³, D’Agostino S.³, D’Auria F.⁴, Traficante A.⁴, Sgambato A.⁵, D’Arena G.⁶
1. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Clinical Research and Advanced Diagnostics, Rionero in Vulture, Italy-luciana.valvano@crob.it; teodora.statuto@crob.it;
2. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Pre-Clinical and Translational Research, Rionero in Vulture, Italy-giovanni.calice@crob.it;
3. Hematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, Italy - giuseppe.pietrantuono@crob.it - giovanna.mansueto@crob.it - oreste.villani@crob.it -
Luanamarano@libero.it - simona.dagostino@crob.it;
4. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Clinical Pathology, Rionero in Vulture, Italy-fiorella.dauria@crob.it;antonio.traficante@crob.it;
5. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Scientific Direction, Rionero in Vulture, Italy-alessandro.sgambato@crob.it;
6. “S. Luca” Hospital, Hematology ASL Salerno, Italy- g.darena@aslsalerno.it;
* These authors contributed equally to the paper.Sessione Plenaria Multidisciplinare

“APPLICAZIONI IN IMMUNOLOGIA ED EMATOLOGIA”
Moderatori: Alessandra Battaglia (Roma), Rosa Chianese (Varese)

15.00
Diagnosi differenziale delle sindromi linfoproliferative croniche
Giovanni D’Arena (Salerno)

15.20
Adaptive immunity to SARS-CoV-2 infection and response to mRNA vaccine
Francesco Annunziato (Firenze)

15.40
Discussione

Moderatori: Andrea Fattorossi (Roma), Igea D’Agnano (Milano)

15.50
Rilevazione ad altissima sensibilità di vescicole extracellulari ed exosomi per biopsie liquide
Alessandro Serra
Supported by LUMINEX

16.05
Discussione

16.10
Lo studio della malattia residua minima nelle leucemie acute pediatriche: l’impatto nella gestione clinica e nella cura del paziente
Barbara Buldini (UniPadova)
Supported by BD BIOSCIENCES

16.25
Discussione

RILEVANZA DELLA CITOMETRIA A FLUSSO NELLA VALUTAZIONE DELLE LEUCEMIE ACUTE MIELOIDI
Moderatori: Rachele Amodeo (Roma), Silvestro Volpe (Avellino)

16.30
Approcccio alla MRD delle LAM
Giovanni Rossi (San Giovanni Rotondo)

16.50
Discussione

16.55 - 17.15
Comunicazioni orali
EMATOLOGIATHE CONTRIBUTION OF FLOW CYTOMETRY TO THE DIAGNOSIS OF AGGRESSIVE B CELL LYMPHOMA: ANALYSYS OF 97 LYMPH NODE BIOPSIES. A SINGLE CENTER EXPERIENCE
Bellesi S.¹, Maiolo E.¹, Alma E.¹, Fatone F.¹, Puggioni PL.¹, Marchionni F.¹, D’Innocenzo S.¹, Contegiacomo A.¹, Attili F.², Potenza A.², D’Alò F.¹, Larocca LM.³, Hohaus S.¹
1. Dip.di Diagnostica per Immagini, Radioterapia Oncologica ed Ematologia Fondazione Policlinico
Univ. A. Gemelli, IRCCS Roma;
2. Dipartimento di Scienze Mediche e Chirurgiche, Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma;
3. Divisione di Anatomia Patologia e Istologia, Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma;

HODGKIN (HL), FOLLICULAR (FL) AND DIFFUSE LARGE B CELL (DLBCL) LYMPHOMAS MICROENVIROMENT : CD26, CD38 AND CD39 ANALYSIS BY FLOW CYTOMETRY (FC)
Rosa Di Gaetano^, Donata Belvini^, Maria Angela De Benedetto^*, Vasileios Mourmouras^**, Cristina Tassinari^*, Antonio Scapinello^**
* U.O.S.D. Transfusional Center, Castelfranco Veneto, AULSS2-Veneto;
Pathology Unit, IOV Castelfranco Veneto.

NUOVE FRONTIERE IN ONCOLOGIA: EVENTI RARI E BIOPSIA LIQUIDA
Moderatori: Giuseppe Pirozzi (Napoli), Virginia Tirino (Napoli)

17.15
L’analisi del cfDNA nella gestione del paziente oncologico
Nicola Normanno (Napoli)

17.35
Discussione

17.40 - 18.00
Comunicazioni orali
ONCOLOGIAMULTIPARAMETRIC FLOW CYTOMETRY HIGHLIGHTS B7-H3 AS A POSSIBLE NOVEL DIAGNOSTIC/THERAPEUTIC TARGET IN GD2NEG/LOW NEUROBLASTOMA VARIANTS
Alessandra Dondero¹, Martina Morini², Davide Cangelosi³, Katia Mazzocco⁴, Martina Serra⁵, Grazia Maria Spaggiari¹, Annalisa Tondo⁶, Franco Locatelli⁷, Aurora Castellano⁷, Francesca Scuderi⁸, Angela Rita Sementa⁴, Alessandra Eva², Massimo Conte⁸, Alberto Garaventa⁸, Cristina Bottino^1,5 and Roberta Castriconi¹
1. Dipartimento di Medicina Sperimentale, Università di Genova, Genova, Italy, alessandra.dondero@unige.it;
2. Laboratory of Molecular Biology, IRCCS Istituto G. Gaslini, Genova, Italy, martinamorini@gaslini.org;
3. Clinical Bioinformatic Unit, IRCCS Istituto Giannina Gaslini, Genova, Italy, davidecangelosi@gaslini.org;
4. UOC Anatomia Patologica, Istituto G. Gaslini, Genova, Italia, katiamazzocco@gaslini.org;
5. Laboratorio di Immunologia Clinica e Sperimentale, IRCCS Istituto G. Gaslini, Genova, Italia, smarti25@infinito.it;
6. Oncohematology Unit, Department of Pediatric Oncology, A. Meyer Children’s University Hospital, Florence, Italy,
annalisa.tondo@meyer.it;
7. Department of Pediatric Hematology and Oncology, Istituto di Ricovero e Cura a Carattere Scientifico Ospedale
Pediatrico Bambino Gesù, Rome, Sapienza, University of Rome, Italy, franco.locatelli@opbg.net,
aurora.castellano@opbg.net;
8. UOC Oncology, IRCCS Istituto Giannina Gaslini, Genoa, Italy, massimoconte@gaslini.org,
albertogaraventa@gaslini.org

A DIETARY GANODERMA LUCIDUM-BASED SUPPLEMENTATION AS A CHEMOTHERAPEUTIC ADJUVANT THERAPY IN GLIOBLASTOMA**
Inguscio C.R.¹, Ferrari B.¹, Priori E.C.¹, De Luca F.¹, Ratto D.², Di Iorio C.², Veneroni P.¹, Desiderio A.³, Angelone P.², Mazzini G.⁵, Ravera M.⁶, Savino E.³, Roda E.^1,4, Rossi P.³ and Bottone M.G.¹
1. Cell Biology & Neurobiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
2. Neurophysiology & Integrated Phys. Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
3. Mycology Lab, Earth & Environmental Science Dept (DSTA), UniPV, Italy;
4. Clinical & Experimental Toxicology Lab, Pavia Poison Center-CNIT, Toxicology Unit, ICS Maugeri SpA,
5. Institute of Molecular Genetics, CNR, Pavia, Italy;
6. Sciences & Technological Innovation Dept, UniPO, Alessandria, Italy;
7. E-mail: chiararita.inguscio01@universitadipavia.it;

Martedì 15 giugno

14.00 - 14.30
Sessione di Presentazione e Discussione Comunicazioni Poster Immunologia
Moderatori: Chiara Agrati (Roma), Claudio Pioli (Roma)

SPIKE-SPECIFIC IMMUNE RESPONSE INDUCED BY BNT162B2 MRNA VACCINE IN FORMER COVID-19 PATIENTS AND HIGH RESPONSIVE SUBJECTS
Elisabetta De Santis¹, Marta Cocciolo¹, Mattia Miroballo¹, Francesco Tamiro¹, Beatrice Totti¹, Giovanni Rossi¹, Ada Piepoli¹, Gabriella De Vincentis¹, Antonio Greco¹, Alessandra Mangia¹, Lazzaro Di Mauro¹, Giuseppe Miscio^1* & Vincenzo Giambra^1*
1. Fondazione IRCCS “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo (FG), Italy
* Equal contribution

BASOPHIL CELL ACTIVATION TEST BY FLOW CYTOMETRY IN THE MANAGEMENT OF IMMEDIATE DRUG HYPERSENSITIVITY TO CARBOPLATIN IN OVARIAN CANCER PATIENTS
Fossati M.¹, La Sorda M.², Ferrairoli M.², Buzzonetti A.¹, Zampetti N.³, Sanguinetti M.², Fattorossi A.³, Battaglia A.⁴
1. Dipartimento Scienze della Salute della Donna, del Bambino e di Sanità Pubblica, Fondazione Policlinico Universitario
A. Gemelli, IRCCS, Roma, Italia;
2. Istituto di Microbiologia Fondazione Policlinico Universitario Agostino Gemelli IRCCS-Roma, Italia;
3. Fondazione Policlinico Universitario A.Gemelli IRCCS, Facility of Cytometry, ROMA;
4. Università Cattolica del Sacro Cuore di Roma, Lab Immunologia e Citometria, Dipartimento Scienze della Vita e Sanità Pubblica
Marco.Fossati@policlinicogemelli.it

PHENOTYPIC AND FUNCTIONAL HETEROGENEITY OF LOW-DENSITY AND HIGH-DENSITY HUMAN LUNG MACROPHAGES
Scalia G.⁵, Balestrieri B.¹, Granata F.^1,2, Petraroli A.^1,2, Massimo Triggiani⁷, Stefania Loffredo^1,2,3,4
1. Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy
stefanialoffredo@hotmail.com (S.L.) - petrarol@unina.it (A.P.) - frapagra@hotmail.com;
2. Center of Excellence, World Allergy Organization (WAO), 80131 Naples, Italy;
3. Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy;
4. Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy;
5. Clinical and Experimental Cytometry Unit, CEINGE-Biotecnologie Avanzate, 80131 Naples, Italy; scalia@ceinge.unina.it;
7. Division of Allergy and Clinical Immunology, University of Salerno, 84084 Fisciano (SA), Italy; mtriggiani@unisa.it (M.T.)

A CASE OF REFRACTORY CYTOPENIA AND INFLAMMATORY SYNDROME CAUSED BY HETEROZYGOUS MUTATION OF AIRE GENE: IS GENETICS ALWAYS RIGHT?
Ebe Schiavo¹, Beatrice Martini¹, Sara Ciullini Mannurita², Laura Luti³, Claudio Favre², Eleonora Gambineri^1,2
1. Department of NEUROFARBA, University of Florence, Florence, Italy;
2. Pediatric Hematology/Oncology Department, Meyer Children’s University Hospital, Florence, Italy;
3. Pediatric Hematology/Oncology Unit, A.O.U. Pisana, Pisa, Italy.

TCRγδ+ AND IL4+ T CELLS AS BIOMARKERS OF MUCOSAL LESION IN CELIAC DISEASE
Serena Vitale¹, Mariantonia Maglio², Stefania Picascia¹, Ilaria Mottola¹, Erasmo Miele², Renata Auricchio², Riccardo Troncone^1,2, Carmen Gianfrani^1,2
1. Institute of Biochemistry and Cell Biology - CNR, Naples;
2. Department of Translational Medicine & European Laboratory for the Investigation of Food-Induced Diseases,
University Federico II, Naples, ITALY - serena.vitale@ibbc.cnr.it

SESSIONE PLENARIA MULTIDISCIPLINARE
APPLICAZIONI IN EMATOLOGIA ED IMMUNOLOGIA
Moderatori: Alessandra Battaglia (Roma), Giovanni D’Arena (Salerno)

14.30
Caratterizzazione immunofenotipica del compartimento sinoviale e periferico per la medicina di precisione nell’Artrite Reumatoide
Stefano Alivernini (Roma)

14.50
Malattia minima residua nelle leucemie acute linfoblastiche nell’epoca della immunoterapia con CAR-T
Giuseppe Gaipa (Monza)

15.10
Discussione

LA VALUTAZIONE CITOMETRICA DELLA TERAPIA DEI PAZIENTI EMATOLOGICI
Moderatori: Giovanni D’Arena (Salerno), Silvestro Volpe (Avellino)

15.20
Cytometric monitoring of chronic lymphoproliferative disease treated with targeted therapies
Andrew C. Rawstron (Leeds-UK)

15.40
Discussione

15.45 - 15-55
Comunicazioni orali EMATOLOGIA

FLOW-CYTOMETRIC IDENTIFICATION OF HODGKIN REED-STERNBERG CELLS AND ASSESSMENT OF PD-L1 EXPRESSION IN LYMPH NODE CELL SUSPENSIONS
Bellesi S.¹, Maiolo E.¹, Alma E.¹, Fatone F.¹, Viscovo M.¹, Puggioni PL.¹, Marchionni F.¹, D’Innocenzo S.¹, Meacci E.², Corina L.³, Fiorita A.³, D’Alò F.¹, Larocca LM.⁴, Hohaus S.¹
1. Dipartimento di Diagnostica per Immagini, Radioterapia Oncologica ed Ematologia Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma;
2. Dip. di Scienze Mediche e Chirurgiche, Chirurgia Toracica, Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma;
3. Dip. di Scienze dell’invecchiamento, neurologiche, ortopediche e della testa-collo, Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma;
4. Divisione di Anatomia Patologia e Istologia, Fondazione Policlinico Universitario A. Gemelli, IRCCS Roma.

APPLICAZIONI DELLA CITOMETRIA NELLE PATOLOGIE IMMUNO-MEDIATE
Moderatori: Daniela Fenoglio (Genova), Loris Zamai (Urbino)

16.00
Decifrare lo stato di silenzio immunitario in pazienti critici COVID-19
Francesco De Sanctis (Verona)

16.20
Discussione

16.25 - 17.00
Comunicazioni orali IMMUNOLOGIA

DIRECT SORTING OF RECOMBINANT MVA BY FLOW VIROMETRY
Di Terlizzi S.^b, Panigada M.a, Boselli D.^b, Romanò M.^b, Canonico E^.b, Villa C.^b, Soprana E.^a, Siccardi AG.^a
a. Molecular Immunology Unit, San Raffaele Scientific Institute, Milan, Italy;
b. FRACTAL - San Raffaele Scientific Institute, Milan, Italy.

DEVELOPMENT OF EXHAUSTION AND ACQUISITION OF REGULATORY FUNCTION BY INFILTRATING CD8+CD28- T LYMPHOCYTES DICTATE CLINICAL OUTCOME IN HEAD AND NECK CANCER
Fenoglio D.^1,2, Belgioia L.^3,4, Parodi A.¹, Missale F.^5,6, Bacigalupo A.^3,4, Tarke A.¹, Negrini S.^1,8, Vecchio S.¹⁰, Altosole T.¹, Vlah S.¹, Astone G.¹, Costabile F.¹, Ascoli A.^6,10, Ferrera F.¹, Schenone G.⁶, De Palma R.^1,8, Signori A.¹¹, Peretti G.^6,10, Corvò R.^3,4, and Filaci G.^1,2
1. Excellence Centre (CEBR), University of Genoa;
2. Bioterapy Unit, IRCCS Ospedale Policlinico San Martino;
3. Dep of Health Science, University of Genoa;
4. Radiation Oncology Unit, IRCCS Ospedale Policlinico San Martino;
5. Dep of Molecular and Translational Medicine, University of Brescia;
6. Unit of Otorhinolaryngology, IRCCS Ospedale Policlinico San Martino;
8. Dep of Internal Medicine, University of Genoa;
9. Medical Oncology Unit, IRCCS Ospedale Policlinico San Martino;
10. Dep of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa;
11. Biostatistics Unit, Dep of Health Science, University of Genoa.

IDENTIFICATION OF FUNCTIONAL SUBSETS OF T LYMPHOCYTES BASED ON THE EXPRESSION OF IMMUNE-CHECKPOINTS, IN JUVENILE IDIOPATHIC ARTHRITIS
Anna Vanni¹, Alessio Mazzoni¹, Laura Maggi¹, Manuela Capone¹, Roberto Semeraro², Giulia Lamacchia¹, Lorenzo Salvati, Alberto Magi², Rolando Cimaz³, Lorenzo Cosmi^1,4, Francesco Liotta^1,4, Francesco Annunziato^1,5
1. Department of Experimental and Clinical Medicine;
2. Department of Information EngineeringUniversity of Florence, Florence, Italy;
3. Department of Clinical Sciences and Community Health, and RECAP-RDUniversity of Milan, Milan, Italy;
4. Immunology and Cell Therapy Unit;
5. Flow Cytometry Diagnostic Center and Immunotherapy (CDCI), Careggi University Hospital, Florence, Italy.

B CELL RELATED PREDICTIVE BIOMARKERS OF TREATMENT RESPONSE IN MYASTHENIA GRAVIS
Valentina Damato¹, Gregorio Spagni¹, Gabriele Monte¹, Alessandra Battaglia², Marco Fossati³, Alexia Buzzonetti³, Nicole Zampetti⁴, Andrea Fattorossi⁴, Amelia Evoli¹
1. Dipartimento di Neuroscienze, Università Cattolica del Sacro Cuore di Roma, Rome, Italy;
2. Dipartimento Scienze della Vita e Sanità Pubblica, Lab.o di immunologia e Citometria, Università Cattolica
del Sacro Cuore di Roma, Rome, Italy;
3. Dipartimento Scienze della Salute della Donna, del Bambino e di Sanità Pubblica, Fondazione Policlinico
Universitario A. Gemelli IRCCS, Rome, Italy;
4. Facility of Flow Cytometry, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy;

17.00 - 18.00
WORKSHOP MULTIDISCIPLINARE BECKMAN COULTER
Applicazioni dell’analisi multiparametrica in Citometria a flusso
Moderatori: Raffaele De Vita (Roma), Giuliano Mazzini (Pavia)

Storia e innovazione in Citometria a flusso
Marcello Bertoletti

Terapia con anti-GD2 CAR-T cells nel trattamento di pazienti pediatrici affetti da neuroblastoma
Valentina Bertaina (IRCCS Osp. Pediatrico Bambino Gesù Roma)

NOTCH4 e Treg nell’infezione da COVID-19
Paola Contini (Di.M.I. Università di Genova)

Mercoledì 16 giugno

Sessione di Presentazione e Discussione

14.00 - 14.40
Comunicazioni Poster Oncologia e Metodologie e Strumentazione
Moderatori: Giuliano Mazzini (Pavia), Cesare Usai (Genova)

THE NUCLEAR ENVELOPE: A TOLLBOOTH FUNCTION FOR SPLICING FACTORS
Casali C.¹, Zannino L.¹, Biggiogera M.¹
1. Laboratory of Cell Biology and Neurobiology, Department of Biology and Biotechnology, University of Pavia, Italy - claudio.casali@unipv.it

NEW MOLECULAR TARGETS FOR OVERCOMING DRUG RESISTANCE IN TRIPLE NEGATIVE BREAST CANCER
De Luca F.¹, Di Iorio C.², Ratto D.², Priori E.C.^1,2, Favaron C.¹, Inguscio C.R.¹, Ferrari B.¹, Savino E.³, Roda E.^1,4, Bottone M.G.¹ and Rossi P.²
1. Cell Biology & Neurobiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
2. Neurophysiology & Integrated Physiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
3. Mycology Lab, Earth & Environmental Science Dept, DSTA, UniPV, Italy;
4. Clinical & Experimental Toxicology Lab, Pavia Poison Centre, CNIT, Toxicology Unit, ICS Maugeri Spa, IRCCS Pavia, Italy;
fabrizio.deluca01@universitadipavia.it

FLOW-CYTOMETRY APPLICATIONS TO CHARACTERIZE THE RADIATION RESPONSE OF COLORECTAL ADENOCARCINOMA CELLS.
Lonati L.¹, Guardamagna I.¹, Ottolenghi A.¹, Baiocco G.¹
1. Lab. Radiobiologia e Biofisica delle Radiazioni, Dipartimento di Fisica, Università di Pavia, Italy - leonardo.lonati01@universitadipavia.it

OXIDATIVE STRESS IN VIABLE SPERMATOZOA: DETECTION IN NATIVE SEMEN SAMPLES
Oumaima Ammar^b, Lucrezia Riley^a, Tommaso Mello^b, Lisa Giovannelli^c, Linda Vignozzi^b, Monica Muratori^b*
a. Data Medica, Via della Salute, 1 Montecatini Terme, Pistoia, Italy;
b. Dep. of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Viale G. Pieraccini 6, Florence, Italy;
c. Department NEUROFARBA, University of Florence, Viale G. Pieraccini 6, Florence, Italy;
Corresponding author: Oumaima Ammar, Department of Experimental and Clinical Biomedical Sciences, “Mario Serio”, University of Florence, Florence Viale Pieraccini, 6 I-50139 Firenze Italy Tel: +39 333 953 2704;
e-mail: ammaroumayma2014@gmail.com

OXIDATIVE STRESS AND NEUROINFLAMMATION EFFECTS IN HIPPOCAMPAL DEVELOPMENT, AGEING, AND DISEASE
Priori E.C.¹, De Luca F.¹, Inguscio C.R.¹, Ferrari B.^1/, Ratto D.¹, Di Iorio C.¹, Desiderio A.¹, Angelone P.¹, Savino E.², Bottone M.G.¹, Rossi P.¹ and Roda E.^1,3
1. Cell Biology & Neurobiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
2. Neurophysiology & Integrated Phys. Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
3. Clinical & Experimental Toxicology Lab, Pavia Poison-CNIT, Toxicology Unit, ICS Maugeri Spa, IRCCS Pavia, Italy;
4. Mycology Lab, Earth & Environmental Sciences Dept (DSTA), UniPV, Italy
ericacecilia.priori01@universitadipavia.it

IONIZING RADIATIONS INDUCE MITOCHONDRIAL IMPAIRMENT IN NEUROBLASTOMA CELL LINES
Tortolici Flavia^, Incocciati Bernadette^, Aquilano Katia^, Lettieri-Barbato Daniele^, Giovanetti Anna^°, Rufini Stefano*
Department of Biology, University of Rome “Tor Vergata”;
°ENEA, Department of Energy and Sustainable Economic, Rome, Italy

KNOCKING DOWN THE METABOTROPIC GLUTAMATE RECEPTOR 5 IN A MOUSE MODEL OF AMIOTROPHIC LATERAL SCLEROSIS REDUCES THE REACTIVE PHENOTYPE OF EX-VIVO CULTURED SPINAL CORD ASTROCYTES
Usai C.¹, Provenzano F.², Gallia E.², Milanese M.^2,3, Bonifacino T.², Bonanno G.^2,3
1. Institute of Biophysics, National Research Council Genoa, Italy;
2. Department of Pharmacy, Unit of Pharmacology and Toxicology, University of Genoa, Italy;
3. Centre of Excellence for Biomedical Research, University of Genoa, Italy.

SESSIONE PLENARIA MULTIDISCIPLINARE
QUANDO LA CITOMETRIA È DECISIVA IN ONCOLOGIA E MICROBIOLOGIA
Moderatori: Stefano Amalfitano (Roma), Virginia Tirino (Napoli)

14.40
Il melanoma e nuove frontiere nell’immunoterapia
Michele Maio (Siena)

15.00
Citometria e Next Generation Sequencing: una sinergia nello studio del danno genomico nella tumorigenesi
Mario Faretta (Milano)

15.20
Computational analysis of microbial flow cytometry data
Ruben Props (Ghent University)

15.40
Discussione

Moderatori: Andrea Fattorossi (Roma), Giuliano Mazzini (Pavia)

15.50
Applicazioni di citofluorimetria per la caratterizzazione di cellule staminali multipotenti: determinare l’identità e il destino delle cellule staminali per scopi rigenerativi
Silvia Barbon (UniPadova)
Supported by SYSMEX

16.05
Discussione

16.10
Uno sguardo ad ampio spettro sul futuro della citometria a flusso
Enrico Ghersi
Supported by CAMPOVERDE

16.25
Discussione

16.30
Piattaforma di Imaging per l’identificazione di nuovi potenziali bersagli di terapie CAR in tumori solidi
Dario Leone
Supported by MILTENYI BIOTEC

16.45
Discussione

16.50
T-cell immunotherapy research
Reyna Alejandra Jiménez (Cytognos)
Supported by PRODOTTI GIANNI

17.05
Discussione

17.10
Design and Engineering of Open Architecture, Triple UV/Violet
Analyzer for High Sensitive and Label Free Cytometry
Paolo Cappella
Supported by CYTOFLOW

17.25
Discussione

17.30 - 18.00
Comunicazioni orali ONCOLOGIA
Moderatori: Giuseppe Pirozzi (Napoli), Virginia Tirino (Napoli)

CHARACTERIZATION OF SMALL EXTRACELLULAR VESICLES SPECIFICALLY SECRETED BY HUMAN TUMOR CELLS IN ORTHOTOPIC PRECLINICAL MODELS IN VIVO
Cifola I.¹, Gatti G.², Panini N.³, Torcinaro A.³, Vilardo L.¹, Bonaventura F.³, Orsini T.³, De Santa F.³, Raspa M.³ and D’Agnano I.¹
1. CNR, Institute for Biomedial Technologies, Segrate (MI);
2. BIOMETRA Department, University of Milan;
3. Mario Negri Institute, Milan;
4. CNR, Institute of Biochemistry and Cell Biology, Rome, Italy - igea.dagnano@cnr.it

VOACAMINE, BISINDOLE ALKALOID, ACTS AS CHEMOSENSITIZING AGENT ON MULTIDRUG RESISTANT TUMOR CELLS OVEREXPRESSING P-GP
Condello M.¹, Meschini S.¹
1. Istituto Superiore di Sanità, Centro Nazionale per la Ricerca e la Valutazione preclinica e clinica dei Farmaci, Roma, Italia-maria.condello@iss.it

RESPONSIVE NANOPARTICLES FOR EFFICIENT GENE DELIVERY IN IMMUNO-ONCOLOGY
Ilaria E. Palamà^1, Clara Guido², Gabriele Maiorano¹, Giuseppe Gigli^1,2
1. CNR-Istituto di Nanotecnologia- Italy - ilaria.palama@nanotec.cnr.it;
2. Dipartimento di Matematica e Fisica, Università Del Salento, Italy.

Giovedì 17 giugno

SESSIONE PLENARIA

COVID-19: IL CONTRIBUTO DELLA CITOMETRIA NELLA RICERCA E NELLA CLINICA
Moderatori: Daniela Fenoglio (Genova), Claudio Pioli (Roma)

14.00
Identificazione di epitopi T in convalescenti COVID-19 tramite tecniche di citometria flusso su larga scala
Alba Grifoni (La Jolla-USA)

14.20
Caratteristiche della risposta immunitaria all’infezione da SARS-CoV-2
Sara De Biasi (UniModena)
Supported by FLUIDIGM

14.35
Discussione

Moderatori: Raffaele De Vita (Roma), Laura Maggi (Firenze)

14.45
Cellule soppressorie di derivazione mieloide nell’infezione da SARS-CoV-2: ruolo nella patogenesi
Chiara Agrati (Ist. Spallanzani Roma)
Supported by BECKMAN COULTER

15.00
Discussione

15.05
Rilevazione citometrica di anticorpi anti-COVID 19 con un nuovo SARS-CoV-2 Multi-Antigen Antibody Kit
Alessandro Serra
Supported by LUMINEX

15.20
Discussione

15.25
L’esperienza della COVID Unit di Siena. Studio prospettico per il monitoraggio di biomarker utili alla migliore gestione del paziente ospedalizzato
Laura Bergantini, Miriana d’Alessandro (UniSiena)
Supported by BD BIOSCIENCES

15.40
Discussione

15.45 - 16.30
Comunicazioni orali COVID-19
Moderatori: Daniela Fenoglio (Genova), Claudio Pioli (Roma)

THE ASSESSMENT OF CD8+ DR+ T LYMPHOCYTES CORRELATES WITH THE SEVERITY OF COVID-19 PATIENTS. A POTENTIAL THERAPEUTIC TARGET TO HYPERIMMUNE PLASMA THERAPY
Alfieri G., Sica C., Giliberti A., Volpe S.
AORN San Giuseppe Moscati - Avellino

CD169 EXPRESSION ON MONOCYTES WAS INVOLVED IN SARS-COV-2 INFECTION AND WAS ASSOCIATED TO CLINICAL FEATURES OF COVID-19
Fanelli M.¹, Petrone V.¹, Minutolo A.¹, Iannetta M.^2,3, Giudice M.¹, Rasi G.¹, Sinibaldi Vallebona P.^1,4, Sarmati L.^2,3, Andreoni M.^2,3, Malergue F.⁵, Balestrieri E.¹, Grelli S.^1,6, Matteucci C.¹
1. Department of Experimental Medicine, University of Rome Tor Vergata, Rome, 00133, Italy;
2. Department of Systems Medicine, University of Rome Tor Vergata, Rome, 00133, Italy;
3. Infectious Diseases Clinic, Policlinic of Tor Vergata, Rome, 00133, Italy;
4. Institute of Translational Pharmacology, National Research Council, Rome, 00133, Italy;
5. Department of Research and Development, Immunotech-Beckman Coulter, Marseille, France;
6. Virology Unit, Policlinic of Tor Vergata, Rome, 00133, Italy.

CHARACTERIZATION OF T LYMPHOCYTES IN SEVERE COVID-19 PATIENTS
Fenoglio D.^1,2, Dentone C.³, Parodi A.², Di Biagio A.^3,4, Bozzano F.³, Vena A.³, Fabbi M.², Ferrera F.¹, Altosole T.¹, Bruzzone B.⁵, Giacomini M.⁶, Pelosi P.^7,8, De Maria A.^1,3,4, Bassetti M.^3,4, De Palma R.⁹, Filaci G.^1,2
1. Excellence Centre for Biomedical Research, University of Genoa;
2. Biotherapy Unit, IRCCS Ospedale Policlinico San Martino;
3. Division of Infectious Diseases, IRCCS Ospedale Policlinico San Martino;
4. Dep of Health Sciences, University of Genoa;
5. Hygiene Unit, IRCCS Ospedale Policlinico San Martino;
6. Dep of Informatics Bioengineering, Robotics, and Systems Engineering (DIBRIS), University of Genoa;
7. Dep of Surgical Sciences and Integrated Diagnostics, University of Genoa;
8. Anesthesia and Intensive Care Unit, IRCCS Ospedale Policlinico San Martino;
9. Internal Medicine, Clinical Immunology and Translational Medicine Unit, IRCCS Ospedale Policlinico San Martino.

CELL-MEDIATED AND HUMORAL ADAPTIVE IMMUNE RESPONSES TO SARS-COV-2 ARE LOWER IN ASYMPTOMATIC THAN SYMPTOMATIC COVID-19 PATIENTS, BOTH DURING INFECTION AND AFTER RECOVERING
Maggi L.¹, Mazzoni A.¹, Capone M.¹, Salvati L.¹, Vanni A.¹, Liotta F.¹, Cosmi L.¹, Annunziato F.¹ and COVID research group 2
1. University of Florence Dept. of Experimental and Clinical Medicine, Florence, Italy;
2. AOUCareggi Hospital, Florence, Italy.

EXPRESSION OF THE PATHOGENIC HERV-W ENVELOPE IN T LYM PHOCYTES IN ASSOCIATION WITH THE RESPIRATORY OUTCOME OF COVID-19 PATIENTS
Petrone V.¹, Fanelli M.¹, Minutolo A.¹, Iannetta M.^2,3, Sinibaldi Vallebona P.^1,4, Sarmati L.^2,3, Andreoni M.^2,3, Perron H.^5,6, Balestrieri E.¹, Grelli S.^1,7, Matteucci C. ¹
1. Department of Experimental Medicine, University of Rome Tor Vergata, Rome, 00133, Italy;
2. Department of Systems Medicine, University of Rome Tor Vergata, Rome, 00133, Italy;
3. Infectious Diseases Clinic, Policlinic of Tor Vergata, Rome, 00133, Italy;
4. Institute of Translational Pharmacology, National Research Council, Rome, 00133, Italy;
5. Geneuro - Innovation, Lyon-France 69008;
6. University of Lyon, Lyon, 69007, France;
7. Virology Unit, Policlinic of Tor Vergata, Rome, 00133, Italy.

17.00 - 18.00
Assemblea dei Soci
- Attività Istituzionali Societarie
- Attività di formazione e aggiornamento
- Premi di Studio GIC
- Premi Poster Citometria

Venerdì 18 giugno

2nd WORKSHOP EXOFLOWMETRY - GIC
“The Many Faces of Flow Cytometry in Microbiology, Environment, Food and Biotechnology”

13.00 - 14.00
SELECTED POSTER PRESENTATIONS
Coordinators: Debora Giorgi (ENEA, Rome), Stefano Amalfitano (CNR, Rome)

SEMI-AUTOMATED, PULSE-SHAPE SCANNING FLOW CYTOMETRY FOR THE MONITORING OF PHYTOPLANKTON AT FINE SPATIO-TEMPORAL SCALES
Epinoux A.¹, van Dijk M.¹, Amaral-Zettler L.^2,3, Zettler.², Artigas L.F.⁴, Bernardi-Aubry F.⁵, Casotti R.¹
1,4. Stazione Zoologica Anton Dohrn, Napoli;
2. NIOZ Royal Netherlands Institute for Sea Research, ‘t Horntje, the Netherlands;
3. Institute for Biodiversity and Ecosystem Dynamics, Amsterdam, the Netherlands;
4.CNRS UMR8187 LOG, MREN-Université du Littoral, Wimereux, France;
5.CNR-ISMAR Venezia, Italy
alexandre.epinoux@szn.it

ANTIBODY-BASED FLOW CYTOMETRY METHOD FOR THE DETECTION OF A. MUCINIPHILA IN COMPLEX CELL SUSPENSIONS
Filardi R.¹, Tytgat H.², Reunanen J.³, De Vos W. M.^2,4, Mora D.1, Arioli S.¹
1. Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy
2. Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
3. Biocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
4. Human Microbiome Research Program Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland
rossella.filardi@unimi.it

AN EFFICENT AND SOLID METHOD FOR A HIGH YIELD EXTRACTION OF NUCLEI FROM MICRO AMOUNT OF FRESH PLANT TISSUE
Metelli G.^1,2, Giorgi D.¹, Farina A.¹, Nardi L.¹, Lucretti S.¹
1. Dept. Sustainable Development and Innovation of the Agro-Industrial System, Biotech Lab, ENEA Casaccia Research Center, Rome, Italy;
2. Dept. Agriculture and Forest Sciences -DAFNE, University of Tuscia, Viterbo, Italy

UNCOVERING THE RELEASE OF MICRO/NANOPLASTICS FROM DISPOSABLE FACE MASKS AT TIMES OF COVID-19
Silvia Morgana¹, Barbara Casentini², Stefano Amalfitano²
1. Institute for the Study of Anthropic Impact and Sustainability in the Marine Environment (IAS-CNR), Via della Vasca Navale, 00146 Rome, Italy
2. Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo (Rome), Italy

WHAT CULTURE-BASED ANALYSES DON’T TELL AND FLOW CYTOMETRY DOES ABOUT VIABLE BUT NON-CULTURABLE MICROORGANISMS
Poscente V.1,3 , Bevivino A.1, Tchuenchieu Kamgain A.2, V., Di Gregorio L.1 , Del Fiore A.¹, Arioli S.⁴, Lucretti S.¹
1. ENEA, Italian Natl. Agency for New Technologies, Energy and Sustainable Economic Dev., Rome, Italy;
2. Ctr. for Food and Nutrition Res., Inst. of Med. Res. and Med.l Plants Studies (IMPM), Yaoundé, Cameroon;
3. Univ. Campus Biomedico of Rome, Rome, Italy;
4. Div. of Food Microbiol. and Bioprocesses, Dept. of Food, Environmental and Nutritional Sci., Univ. of Milan, Milan, Italy

INVITED LECTURES
Coordinators: Stefania Arioli (Univ. Milan, Milan), Cecilia Balestra (OGS, Trieste), Sergio Lucretti (ENEA, Rome)

14.00
ExoFlowMetry: is it real?
Sergio Lucretti (ENEA, Biotechnology Laboratory, Rome, Italy)

14.15
Tips and tricks for a successful isolation of nuclei in plants
João Loureiro (FLOWer Lab, Centre for Functional Ecology, University of Coimbra, Portugal)

14.40
Plant molecular flow cytogenetics
Debora Giorgi (ENEA, Biotechnology Laboratory, Rome, Italy)

15.05
Unravelling chromatin architecture of condensed plant mitotic chromosomes
Petr Capal (Centre of Plant Structural and Functional Genomics, Olomouc, Czech Republic)

15.30
DISCUSSION

15.45
Flow cytometry applied to the study of plant-microbe interactions
Nadia Massa (Science, Technology and Innovation Department - University of Eastern Piedmont “ A. Avogadro”, Alessandria, Italy)
SUPPORTED BY SYSMEX

16.05
How automated flow cytometry can improve our understanding of the plankton structure, distribution and functioning?
Gerarld Gregory et al. (Institut Méditérranéen d’Océanographie, Marseille, France)

16.30
Flow Cytometry in Food Microbiology
Stefania Arioli (Food Science, Nutrition and Environment Dept., University of Milan, Italy)

16.55
Characterization of Extracellular Vesicles in Seminal Liquid
by Nanocytometry
Alberto Alvarez (Univ. de Extremadura, Badajoz, Spain)
SUPPORTED BY BECKMAN COULTER

DISCUSSION

17.15
From CYTO2021 to Exoflowmetry 2, Frontiers in Environmental Flow Cytometry
David W. Galbraith (University of Arizona, AZ, USA)

17.40
DISCUSSION and CONCLUSIONS
David Galbraith, Alberto Alvarez, Sergio Lucretti

18.00
Workshop and Conference closing
Giovanni D’Arena (Salerno), Raffaele De Vita (Roma), Giuliano Mazzini (Pavia), Sergio Lucretti (Roma)

programma

TOPICS

programma

Lunedì 14 giugno

Martedì 15 giugno

Mercoledì 16 giugno

Giovedì 17 giugno

Venerdì 18 giugno

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