POSTER EMATOLOGIA


presentazione lunedì 14 giugno 2021



MORE THAN A SURFACE MARKER: THE RELEVANCE OF CD200 SERUM LEVELS IN PREDICTING PROGNOSIS OF CHRONIC LYMPHOCYTIC
LEUKEMIA
D’Arena G.1, Vitale C.2, Coscia M.2, Lamorte D.3, Pietrantuono G.4, Perutelli F.2, D’Auria F.5, , Valvano T.5, Tomassi S.6, Griggio V.2, Jones R.2, Di Leo A.7, Feola G.8, Guida G.8, Mansueto G.4, Villano O.4, D’Agostino S.4, De Feo V.9, Calapai F.10, Mannucci G.11, Efremov D.12, Laurenti L.6, (giovannidarena@libero.it)
 1. Hematology, P.O. S. Luca, ASL Salerno;
2. Hematology, Department of Molecular Biotechnology and Health Sciences, A.O.U. Città della Salute e della Scienza di Torino, University of Torino;
3. Laboratory of Pre-Clinical and Translational Research, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
4. Hematology and Stem Cell Transplantation Unit, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
5. Laboratory of Clinical and Advanced Diagnostics, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
6. Hematology Unit, Catholic University of Sacred Hearth, Rome, 7. Ortopedics Unit, P.O. S. Luca ASL Salerno;
8. SIT, P.O. S. Luca, ASL Salerno, 9. Department of Pharmaceutical and Biomedical Sciences, University of Salerno;
10. Department of Clinical, Biological and Pharmaceutical in Environmental Sciences, University of Messina:
11. Department of Biomedical, Dental, Morphological and Functional Imaging Sciencies, University of Messina;
12. Molecular Hematology, International Center for Genetic Engineering and Biotechnology, Trieste.



WHAT DOES ATYPICAL CHRONIC LYMPHOCYTIC LEUKEMIA REALLY MEANS? MORPHOLOGY VS IMMUNOPHENOTYPE
D’Arena G.1, D’Auria F.2, Statuto T.2, Valvano T.2, Mansueto G.3, Villani O.3, D’Agostino S.3, Pietrantuono G.3
 1. Hematology, P.O. S. Luca, ASL Salerno;
2. Laboratory of Clinical Research and Advanced Diagnostics, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture;
3. Hematology and Stem Cell Transplantation Unit, IRCCS-CROB Referral Cancer Center of Basilicata, Rionero in Vulture - giovannidarena@libero.it


A NEW NON-INVASIVE METHOD FOR ISOLATION OF CIRCULATING EXTRACELLULAR VESICLES AND EVALUATION OF ITS SUITABILITY FOR
HEMATOLOGICAL MALIGNANCY BIOMARKER DISCOVERY
Laurenzana I.1, Trino S.1, Lamorte D.1, Girasole M.2, Dinarelli S.2, De Stradis A.3, Grieco V.4, Maietti M.5, Traficante A.5, StatutoT.4, Villani O.6, Musto P.6,7, Sgambato A.8, De Luca L.4, Caivano A.4
1. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Preclinical and Translational Research, Rionero in Vulture, PZ, IT;
2. National Research Council (CNR), Institute for the Study of the Structure of Matter, Rome, Italy;
3. National Research Council (CNR), Institute for Sustainable Plant Protection, Bari, Italy;
4. IRCCS-CROB, Laboratory of Clinical Research and Advanced Diagnostics, Rionero in Vulture, PZ, Italy;
5. IRCCS-CROB, Unit of Clinical Pathology, Rionero in Vulture, PZ, Italy;
6. IRCCS-CROB, Hematology and Stem Cell Transplantation Unit, Rionero in Vulture, PZ, Italy;
7. “Aldo Moro” University School of Medicine and Unit of Hematology and Stem Cell Transplantation, Policlinico, Department of Emergency and Organ Transplantation, Bari, Italy
8. IRCCS-CROB, Scientific Direction, Rionero in Vulture, PZ, Italy
ilaria.laurenzana@crob.it


CYTOFLUORIMETRIC ANALYSIS OF TWO BONE MARROW COLLECTION SYSTEMS AND THE IMPORTANCE OF CLINICAL RESULTS
Stefano Papini1, Cristina Borrelli1, Lella Petrella1, Mara Fanelli1, Maria Pia Petrilli1, Pietro Modugno1, Veronica Picone1, Andrea Mazzanti2, Pietro Ornati2, Enrico Maria Centritto1 and Eugenio Caradonna1
1. Gemelli Molise -Campobasso;
2. IRCSS Maugeri - Pavia.



CYTOKINE ANALYSIS IN PLATELET RICH PLASMA OF PATIENTS WITH OSTEOARTHRITIS VS HEALTHY SUBJECTS
Stefano Papini1, Mara Fanelli1, Lella Petrella1, Cristina Borrelli1, Domenico Speciale2, Antonio San Martino2, Emilio Serlenga3, Andrea Mazzanti4, Pietro Ornati4 and Eugenio Caradonna1
1. Gemelli Molise Campobasso;
2. Clinica San Camillo Taranto;
3. Presidio Ospedaliero SS. Annunziata Taranto;
4. IRCSS Maugeri Pavia.



PREMIO DI STUDIO GIC
“GIUSEPPE BASSO”
MUSASHI-2 SUSTAINS THE GROWTH OF MLL-REARRANGED ALL AND IT IS INVOLVED IN GLUCOCORTICOIDS RESISTANCE
G. Passafari12, L. Valsecchi12, M. Mauri2, R. Piazza2, A. Biondi12, G. Cazzaniga12, M. Bardini1
1. Fondazione M. Tettamanti, Clinica Pediatrica, Monza;
2. Università di Milano-Bicocca, Dipartimento di Medicina e Chirurgia, Monza.



PREMIO DI STUDIO GIC “GIUSEPPE BASSO”
FUNCTIONAL CHARACTERIZATION OF GENETIC VARIANTS, IDENTIFIED THROUGH NEXT GENERATION SEQUENCING, WITH A ROLE IN THE PREDISPOSITION TO PEDIATRIC HEMATONCOLOGICAL DISEASES
S. Rebellato, C. Saitta, L. Bettini, G. Giudici, N. Panini, E. Erba, A. Biondi, G. Fazio, G. Cazzaniga
Centro Ricerca Tettamanti, Clinica Pediatrica, Università di Milano Bicocca, Fondazione MBBM, Monza




CIRCULATING REGULATORY T-CELL NUMBER DOES NOT PREDICT PROGNOSIS OF MONOCLONAL GAMMOPATHIES OF UNCERTAIN SIGNIFICANCE
Valvano L.1*, Calice G. 2*, Statuto T.1, Pietrantuono G.3, Mansueto G.3, Villani O.3, Marano L.3, D’Agostino S.3, D’Auria F.4, Traficante A.4, Sgambato A.5, D’Arena G.6
1. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Clinical Research and Advanced Diagnostics, Rionero in Vulture, Italy-luciana.valvano@crob.it; teodora.statuto@crob.it;
2. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Pre-Clinical and Translational Research, Rionero in Vulture, Italy-giovanni.calice@crob.it;
3. Hematology and Stem Cell Transplantation Unit, Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Rionero in Vulture, Italy - giuseppe.pietrantuono@crob.it - giovanna.mansueto@crob.it - oreste.villani@crob.it -
Luanamarano@libero.it - simona.dagostino@crob.it;
4. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Laboratory of Clinical Pathology, Rionero in Vulture, Italy-fiorella.dauria@crob.it;antonio.traficante@crob.it;
5. Centro di Riferimento Oncologico della Basilicata (IRCCS-CROB), Scientific Direction, Rionero in Vulture, Italy-alessandro.sgambato@crob.it;
6. “S. Luca” Hospital, Hematology ASL Salerno, Italy- g.darena@aslsalerno.it;
* These authors contributed equally to the paper.Sessione Plenaria Multidisciplinare



POSTER IMMUNOLOGIA


presentazione martedì 15 giugno 2021



SPIKE-SPECIFIC IMMUNE RESPONSE INDUCED BY BNT162B2 MRNA VACCINE IN FORMER COVID-19 PATIENTS AND HIGH RESPONSIVE SUBJECTS
Elisabetta De Santis1, Marta Cocciolo1, Mattia Miroballo1, Francesco Tamiro1, Beatrice Totti1, Giovanni Rossi1, Ada Piepoli1, Gabriella De Vincentis1, Antonio Greco1, Alessandra Mangia1, Lazzaro Di Mauro1, Giuseppe Miscio1* & Vincenzo Giambra1*
1. Fondazione IRCCS “Casa Sollievo della Sofferenza”, 71013 San Giovanni Rotondo (FG), Italy
* Equal contribution



BASOPHIL CELL ACTIVATION TEST BY FLOW CYTOMETRY IN THE MANAGEMENT OF IMMEDIATE DRUG HYPERSENSITIVITY TO CARBOPLATIN IN OVARIAN CANCER PATIENTS
Fossati M.1, La Sorda M.2, Ferrairoli M.2, Buzzonetti A.1, Zampetti N.3, Sanguinetti M.2, Fattorossi A.3, Battaglia A.4
1. Dipartimento Scienze della Salute della Donna, del Bambino e di Sanità Pubblica, Fondazione Policlinico Universitario
A. Gemelli, IRCCS, Roma, Italia;
2. Istituto di Microbiologia Fondazione Policlinico Universitario Agostino Gemelli IRCCS-Roma, Italia;
3. Fondazione Policlinico Universitario A.Gemelli IRCCS, Facility of Cytometry, ROMA;
4. Università Cattolica del Sacro Cuore di Roma, Lab Immunologia e Citometria, Dipartimento Scienze della Vita e Sanità Pubblica
Marco.Fossati@policlinicogemelli.it



PHENOTYPIC AND FUNCTIONAL HETEROGENEITY OF LOW-DENSITY AND HIGH-DENSITY HUMAN LUNG MACROPHAGES
Scalia G.5, Balestrieri B.1, Granata F.1,2, Petraroli A.1,2, Massimo Triggiani7, Stefania Loffredo1,2,3,4
1. Department of Translational Medical Sciences, University of Naples Federico II, 80131 Naples, Italy
stefanialoffredo@hotmail.com (S.L.) - petrarol@unina.it (A.P.) - frapagra@hotmail.com;
2. Center of Excellence, World Allergy Organization (WAO), 80131 Naples, Italy;
3. Center for Basic and Clinical Immunology Research (CISI), University of Naples Federico II, 80131 Naples, Italy;
4. Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council, 80131 Naples, Italy;
5. Clinical and Experimental Cytometry Unit, CEINGE-Biotecnologie Avanzate, 80131 Naples, Italy; scalia@ceinge.unina.it;
7. Division of Allergy and Clinical Immunology, University of Salerno, 84084 Fisciano (SA), Italy; mtriggiani@unisa.it (M.T.)

A CASE OF REFRACTORY CYTOPENIA AND INFLAMMATORY SYNDROME CAUSED BY HETEROZYGOUS MUTATION OF AIRE GENE: IS GENETICS ALWAYS RIGHT?
Ebe Schiavo1, Beatrice Martini1, Sara Ciullini Mannurita2, Laura Luti3, Claudio Favre2, Eleonora Gambineri1,2
1. Department of NEUROFARBA, University of Florence, Florence, Italy;
2. Pediatric Hematology/Oncology Department, Meyer Children’s University Hospital, Florence, Italy;
3. Pediatric Hematology/Oncology Unit, A.O.U. Pisana, Pisa, Italy.



TCRγδ+ AND IL4+ T CELLS AS BIOMARKERS OF MUCOSAL LESION IN CELIAC DISEASE
Serena Vitale1, Mariantonia Maglio2, Stefania Picascia1, Ilaria Mottola1, Erasmo Miele2, Renata Auricchio2, Riccardo Troncone1,2, Carmen Gianfrani1,2
1. Institute of Biochemistry and Cell Biology - CNR, Naples;
2. Department of Translational Medicine & European Laboratory for the Investigation of Food-Induced Diseases,
University Federico II, Naples, ITALY - serena.vitale@ibbc.cnr.it

POSTER ONCOLOGIA e METODOLOGIE e STRUMENTAZIONE


presentazione mercoledì 16 giugno 2021


THE NUCLEAR ENVELOPE: A TOLLBOOTH FUNCTION FOR SPLICING FACTORS
Casali C.1, Zannino L.1, Biggiogera M.1
1. Laboratory of Cell Biology and Neurobiology, Department of Biology and Biotechnology, University of Pavia, Italy - claudio.casali@unipv.it

NEW MOLECULAR TARGETS FOR OVERCOMING DRUG RESISTANCE IN TRIPLE NEGATIVE BREAST CANCER
De Luca F.1, Di Iorio C.2, Ratto D.2, Priori E.C.1,2, Favaron C.1, Inguscio C.R.1, Ferrari B.1, Savino E.3, Roda E.1,4, Bottone M.G.1 and Rossi P.2
1. Cell Biology & Neurobiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
2. Neurophysiology & Integrated Physiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
3. Mycology Lab, Earth & Environmental Science Dept, DSTA, UniPV, Italy;
4. Clinical & Experimental Toxicology Lab, Pavia Poison Centre, CNIT, Toxicology Unit, ICS Maugeri Spa, IRCCS Pavia, Italy;
fabrizio.deluca01@universitadipavia.it

FLOW-CYTOMETRY APPLICATIONS TO CHARACTERIZE THE RADIATION RESPONSE OF COLORECTAL ADENOCARCINOMA CELLS.
Lonati L.1, Guardamagna I.1, Ottolenghi A.1, Baiocco G.1
1. Lab. Radiobiologia e Biofisica delle Radiazioni, Dipartimento di Fisica, Università di Pavia, Italy - leonardo.lonati01@universitadipavia.it

OXIDATIVE STRESS IN VIABLE SPERMATOZOA: DETECTION IN NATIVE SEMEN SAMPLES
Oumaima Ammarb, Lucrezia Rileya, Tommaso Mellob, Lisa Giovannellic, Linda Vignozzib, Monica Muratorib*
a. Data Medica, Via della Salute, 1 Montecatini Terme, Pistoia, Italy;
b. Dep. of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Viale G. Pieraccini 6, Florence, Italy;
c. Department NEUROFARBA, University of Florence, Viale G. Pieraccini 6, Florence, Italy;
Corresponding author: Oumaima Ammar, Department of Experimental and Clinical Biomedical Sciences, “Mario Serio”, University of Florence, Florence Viale Pieraccini, 6 I-50139 Firenze Italy Tel: +39 333 953 2704;
e-mail: ammaroumayma2014@gmail.com

OXIDATIVE STRESS AND NEUROINFLAMMATION EFFECTS IN HIPPOCAMPAL DEVELOPMENT, AGEING, AND DISEASE
Priori E.C.1, De Luca F.1, Inguscio C.R.1, Ferrari B.1/, Ratto D.1, Di Iorio C.1, Desiderio A.1, Angelone P.1, Savino E.2, Bottone M.G.1, Rossi P.1 and Roda E.1,3
1. Cell Biology & Neurobiology Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
2. Neurophysiology & Integrated Phys. Lab, Biology & Biotechnology “L. Spallanzani” Dept, UniPV, Italy;
3. Clinical & Experimental Toxicology Lab, Pavia Poison-CNIT, Toxicology Unit, ICS Maugeri Spa, IRCCS Pavia, Italy;
4. Mycology Lab, Earth & Environmental Sciences Dept (DSTA), UniPV, Italy
ericacecilia.priori01@universitadipavia.it


IONIZING RADIATIONS INDUCE MITOCHONDRIAL IMPAIRMENT IN NEUROBLASTOMA CELL LINES
Tortolici Flavia*, Incocciati Bernadette*, Aquilano Katia*, Lettieri-Barbato Daniele*, Giovanetti Anna°, Rufini Stefano*
*Department of Biology, University of Rome “Tor Vergata”;
°ENEA, Department of Energy and Sustainable Economic, Rome, Italy


KNOCKING DOWN THE METABOTROPIC GLUTAMATE RECEPTOR 5 IN A MOUSE MODEL OF AMIOTROPHIC LATERAL SCLEROSIS REDUCES THE REACTIVE PHENOTYPE OF EX-VIVO CULTURED SPINAL CORD ASTROCYTES
Usai C.1, Provenzano F.2, Gallia E.2, Milanese M.2,3, Bonifacino T.2, Bonanno G.2,3
1. Institute of Biophysics, National Research Council Genoa, Italy;
2. Department of Pharmacy, Unit of Pharmacology and Toxicology, University of Genoa, Italy;
3. Centre of Excellence for Biomedical Research, University of Genoa, Italy.

POSTER GIC-ExoFlowMetry


presentazione venerdì 18 giugno 2021


SEMI-AUTOMATED, PULSE-SHAPE SCANNING FLOW CYTOMETRY FOR THE MONITORING OF PHYTOPLANKTON AT FINE SPATIO-TEMPORAL SCALES
Epinoux A.1, van Dijk M.1, Amaral-Zettler L.2,3, Zettler.2, Artigas L.F.4, Bernardi-Aubry F.5, Casotti R.1
1,4. Stazione Zoologica Anton Dohrn, Napoli;
2. NIOZ Royal Netherlands Institute for Sea Research, ‘t Horntje, the Netherlands;
3. Institute for Biodiversity and Ecosystem Dynamics, Amsterdam, the Netherlands;
4.CNRS UMR8187 LOG, MREN-Université du Littoral, Wimereux, France;
5.CNR-ISMAR Venezia, Italy
alexandre.epinoux@szn.it


ANTIBODY-BASED FLOW CYTOMETRY METHOD FOR THE DETECTION OF A. MUCINIPHILA IN COMPLEX CELL SUSPENSIONS
Filardi R.1, Tytgat H.2, Reunanen J.3, De Vos W. M.2,4, Mora D.1, Arioli S.1
1. Department of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy
2. Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands
3. Biocenter Oulu & Cancer and Translational Medicine Research Unit, University of Oulu, Oulu, Finland
4. Human Microbiome Research Program Unit, Faculty of Medicine, University of Helsinki, Helsinki, Finland
rossella.filardi@unimi.it

AN EFFICENT AND SOLID METHOD FOR A HIGH YIELD EXTRACTION OF NUCLEI FROM MICRO AMOUNT OF FRESH PLANT TISSUE
Metelli G.1,2, Giorgi D.1, Farina A.1, Nardi L.1, Lucretti S.1
1. Dept. Sustainable Development and Innovation of the Agro-Industrial System, Biotech Lab, ENEA Casaccia Research Center, Rome, Italy;
2. Dept. Agriculture and Forest Sciences -DAFNE, University of Tuscia, Viterbo, Italy

UNCOVERING THE RELEASE OF MICRO/NANOPLASTICS FROM DISPOSABLE FACE MASKS AT TIMES OF COVID-19
Silvia Morgana1, Barbara Casentini2, Stefano Amalfitano2
1. Institute for the Study of Anthropic Impact and Sustainability in the Marine Environment (IAS-CNR), Via della Vasca Navale, 00146 Rome, Italy
2. Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, 00015 Monterotondo (Rome), Italy


WHAT CULTURE-BASED ANALYSES DON’T TELL AND FLOW CYTOMETRY DOES ABOUT VIABLE BUT NON-CULTURABLE MICROORGANISMS
Poscente V.1,3 , Bevivino A.1, Tchuenchieu Kamgain A.2, V., Di Gregorio L.1 , Del Fiore A.1, Arioli S.4, Lucretti S.1
1. ENEA, Italian Natl. Agency for New Technologies, Energy and Sustainable Economic Dev., Rome, Italy;
2. Ctr. for Food and Nutrition Res., Inst. of Med. Res. and Med.l Plants Studies (IMPM), Yaoundé, Cameroon;
3. Univ. Campus Biomedico of Rome, Rome, Italy;
4. Div. of Food Microbiol. and Bioprocesses, Dept. of Food, Environmental and Nutritional Sci., Univ. of Milan, Milan, Italy

 

Il perdurare della situazione emergenziale ci ha fatto bruscamente ritornare alla realtà “virtuale” che fortunatamente abbiamo avuto occasione di sperimentare con successo nei recenti eventi on line del GIC ovvero i “Webseminars” prima e i Corsi 2020 che hanno avuto comunque una grandissima partecipazione.

 

Il Comitato Organizzatore e il Comitato Scientifico invitano le Aziende Sponsor a partecipare attivamente a questa edizione virtuale.

 

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